To ensure the most accurate results, the purchasing of high-quality equipment is required. Streptococci carry group-specific carbohydrate antigens in their cell walls and after extraction, using a specially developed enzyme preparation, these antigens will agglutinate latex particles coated with the corresponding antibody.
In the Strep-Check kit, the different reagents are coated with the specific antibody and will agglutinate in the presence of the enzymatically-extracted antigen. All the reagents are supplied at optimal dilution for use with all recommended techniques without the need for further dilution or addition.
Another common Latex Agglutination Test is for Staphylococcus aureus, also known as MRSA, which can cause a range of infections in skin, soft tissue, blood and the lungs whch may result in pneumonia, overwhelming sepsis or death, so routine checking with a staph test kit should be undertaken frequently.
By placing a drop of test reagent on the staph test agglutination slide and emulsifying colonies of the suspect organism in the agent, the slide can then be examined for signs of agglutination after a minute.
Brucella and Tularemia are tested for by a standard tube agglutination test adapted to run in microtiter plates. In both procedures, the bacterial cells are incubated in microtiter wells along with patient sera and controls. If antibodies to either Brucella or Tularemia are present, an antigen-antibody complex forms and results in a lattice in the well 1 in the image left. If no specific antibodies are present, no clumping occurs and the antigen settles out to form a button 2 in the image left.
Patient sera are incubated with sensitized particles in microtiter wells and unsensitized gelatin particles in control wells. The test depends on what type of sample is needed.
Saliva Urine Blood Cerebrospinal fluid lumbar puncture The sample is sent to a lab, where it is mixed with latex beads coated with a specific antibody or antigen. Latex agglutination results take about 15 minutes to an hour. How to Prepare for the Test. Why the Test is Performed. What Abnormal Results Mean. If there is an antigen-antibody match, agglutination will occur. The risk level depends on the type of test. Test tubes containing three different reagents with either A, B or Rh antibodies.
The antibodies attach to antigens on the patient's red blood cells if they match. First mix the patient's blood with three different reagents including either of the three different antibodies, A, B or Rh antibodies! Then you take a look at what has happened. In which mixtures has clumping, or agglutination, occurred?
The agglutination indicates that the blood has reacted with a certain antibody and is therefore not compatible with blood containing that kind of antibody. If the blood does not agglutinate, it indicates that the blood does not have the antigens binding the special antibody in the reagent. Start by taking a look at the test tubes containing A and B antibodies. Has the blood agglutinated in either of these two tubes?
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